ep300 activity (MedChemExpress)
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ep300 activity
Ep300 Activity, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p300+activator/p300+Antibody/pmc12828234-94-2-11
Average 95 stars, based on 18 article reviews
Ep300 Activity, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p300+activator/p300+Antibody/pmc12828234-94-2-11
Average 95 stars, based on 18 article reviews
ep300 activity - by Bioz Stars,
2026-09
95/100 stars
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1) Product Images from "A novel dual histone mark reader ZCWPW2 regulates meiotic recombination through lactylation and transcriptional regulation in humans and mice"
Article Title: A novel dual histone mark reader ZCWPW2 regulates meiotic recombination through lactylation and transcriptional regulation in humans and mice
Journal: Nucleic Acids Research
doi: 10.1093/nar/gkag049
Figure Legend Snippet: ZCWPW2 binds to H3K4me3-mediated promoter regions in independence of PRDM9. ( A ) Schematic summary illustrating that ZCWPW2 binds strongly to H3K4me3-marked promoter regions both in the absence and presence of PRDM9. ( B ) GO analysis of genes marked by ZCWPW2 peaks at promoter-associated H3K4me3 sites in HEK293T cells. ( C ) CUT&Tag tracks display ZCWPW2 (W2) binding peaks at promoter regions of genes implicated in DNA repair (e.g. RAD51AP1, RAD51C, MRE11, ATM, BARD1, ZCWPW1 ) and chromosome segregation (e.g. SMC2, RAD21, ANKRD31 ), as well as lactylation modification (e.g. EP300 ). IgG was used as a negative control. ( D ) Confirmation of ZCWPW2 (W2) binding at key genes’ promoter regions by PCR amplification using primers targeting the promoter regions. IgG served as a negative control. ( E ) Relative enrichment of ZCWPW2 binding chromatin fragments was measured by qPCR using primers targeting the promoter regions ( n = 3 independent experiments; Two-tailed Student’s t -test; * P < 0.05; error bars, s.e.m.). ( F ) Relative luciferase activity driven by promoter fragments of RAD51C, RAD51AP1, RAD21, ZCWPW1, MRE11, BARD1, EP300, ANKRD31, SMC2 , and ATM in HEK293T cells co-transfected with increasing amounts of Flag- ZCWPW2 (ZCWPW2-OE, + and ++), compared to the control (empty vector) ( n = 3 independent experiments; two-tailed Student’s t -test; * P < 0.05; error bars, s.e.m.). ( G ) qPCR analysis showing significant downregulation of Rad51c1, Rad51ap1, Rad21, Zcwpw1, Mre11a, Brd1, Ep300, Ankrd31, Smc2 , and Atm mRNA in testes from adult Zcwpw2 KO mice compared to WT mice ( n = 3 biologically independent WT mice and KO mice; Two-tailed Student’s t -test; * P < 0.05; error bars, s.e.m.). ( H ) Bar graphs showing relative mRNA expression levels of key meiosis-related genes in testicular biopsies from the ZCWPW2 mutant patient compared to controls ( n = 3 independent experiments; two-tailed Student’s t -test; * P < 0.05; error bars, s.e.m.).
Techniques Used: Binding Assay, Modification, Negative Control, Amplification, Two Tailed Test, Luciferase, Activity Assay, Transfection, Control, Plasmid Preparation, Expressing, Mutagenesis
Figure Legend Snippet: ZCWPW2 promotes lactylation and stability of recombination-associated proteins through coordination with ZCWPW1 in cultured cells. ( A ) Co-IP analysis of HEK293T cells overexpressed with HA-recombination-associated proteins (HSPA2, SYCP1, and MDC1) and Flag-ZCWPW2, with or without Myc-ZCWPW1, and in the presence or absence of siRNA targeting ZCWPW1 . Bar graph quantifies Kla signal intensity for each condition ( n = 3 independent experiments; two-tailed Student’s t -test; * P < 0.05; error bars, s.e.m.). ( B ) Co-IP analysis (left) for Kla of endogenous Mdc1, Hspa2, and Sycp1 in GC-2 cells after individual or combined knockdown of Zcwpw1 or/and Zcwpw2 using siRNAs. Quantification of Kla levels (right) showed a significant decrease in Kla upon Zcwpw1 or Zcwpw2 knockdown, with the greatest reduction observed upon dual silencing ( n = 3 independent experiments; two-tailed Student’s t -test; * P < 0.05; error bars, s.e.m.). ( C and D ) Co-IP (left) and quantification analysis (right) of Kla on endogenous Mdc1, Hspa2, and Sycp1 in GC-2 cells treated with the EP300 inhibitor A-485, the LDHA inhibitor sodium oxamate, or both (C), and with EP300 activator CTPB or exogenous lactate (D) ( n = 3 independent experiments; two-tailed Student’s t -test; * P < 0.05; error bars, s.e.m.).
Techniques Used: Cell Culture, Co-Immunoprecipitation Assay, Two Tailed Test, Knockdown
Figure Legend Snippet: Proposed schema of PRMD9–ZCWPW1–ZCWPW2 system regulating recombination progression. During meiotic recombination, PRDM9 marks the dual histone methylation of H3K4me3 and H3K36me3 at nucleosomes to define recombination hotspots (i, ii), while ZCWPW1 acts as a reader by recognizing these modifications (iii). In this study, we revealed that ZCWPW2 shows enhanced enrichment at dual histone methylation sites in the presence of PRDM9 and forms a complex with ZCWPW1 to interact with recombination machinery-associated proteins at these sites. Additionally, the ZCWPW1–ZCWPW2 complex interacts with and stabilizes the enzymatic activity of lactate dehydrogenase LDHA, promoting local lactate production. In parallel, ZCWPW2 binds independently to H3K4me3-rich promoter regions, regulating the transcription of meiosis-related genes, as well as that of the lactylation writer EP300 to catalyze lactylation. These two coordinated actions further stabilize the recombination machinery via the lactylation pathway, thereby successfully facilitating DSB repair during recombination progression.
Techniques Used: Methylation, Activity Assay
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The p300 activator (cholera toxin B subunit, CTB; Article Title: α-myosin heavy chain lactylation maintains sarcomeric structure and function and alleviates the development of heart failure Article Snippet: cells were collected 48–72 h after transfection. .. LDHA inhibitor (Galloflavin; Cat#HY-W040118; 10 −5 M; 24 h), p300 inhibitor (C646; Cat#HY-13823; 10 −5 M; 24 h), Immunoprecipitation:Article Title: CD8a antibody-functionalized biomimetic red blood cell membrane ectosomes delivering C646 reverse CD8⁺ T Cell exhaustion via H3K18la histone delactylation in gastric cardia adenocarcinoma Article Snippet: Experimental groupings were defined as follows: (1) sh-NC group, sh-p300#1 group, and sh-p300#2 group; (2) Control (Ctrl) group, Lactate group (CD8 + T cells treated with 20 mM sodium lactate for 24 h), Lactate + p300 inhibitor group (CD8 + T cells treated with 20 mM sodium lactate and 30 μM p300 inhibitor C646 for 24 h), Lactate + p300 activator group (CD8 + T cells treated with 20 mM sodium lactate and 100 μM p300 activator CTB for 24 h), Lactate + sh-NC group, and Lactate + sh-p300 group.30 μM p300 inhibitor C646 for 24 h), Lactate + p300 activator group (CD8 + T cells treated with 20 mM sodium lactate and 100 μM p300 activator CTB for 24 h), Lactate + sh-NC group, and Lactate + sh-p300 group. ... The p300 activator (cholera toxin B subunit, CTB; Article Title: α-myosin heavy chain lactylation maintains sarcomeric structure and function and alleviates the development of heart failure Article Snippet: cells were collected 48–72 h after transfection. .. LDHA inhibitor (Galloflavin; Cat#HY-W040118; 10 −5 M; 24 h), p300 inhibitor (C646; Cat#HY-13823; 10 −5 M; 24 h), |
p300 differential expression; ( F ) The tSNE distribution map of EP300 in various cell types in the scRNA-seq data " width="250" height="auto" />
